GENE EXPRESSION ANALYSIS OF THERAPEUTICALLY ACTIVE ANTIPROLIFERATIVE POLYPHENOLIC FRACTIONS EXTRACTED FROM Rubus ellipticus ON HUMAN CERVICAL CANCER CELLS (HeLa)

R. ellipticus is high in natural antioxidants, which aid in reducing oxidative stress and preventing degenerative diseases including cancer and diabetes. Epigenetic modulation of tumor suppressor genes can reduce protein expression of DNMT and HDAC isomers and reverse the tumorgenicity. Hexane, petroleum ether, chloroform, methanol, and water were used in phytochemical extraction process, which were analyzed qualitatively and quantitatively. Phenols, Flavonoids, Tannins, and Carbohydrates are present in different levels in all extracts. Water and methanolic extracts of R. ellipticus had greatest phenolic content of 32.23 mg/gm and 8.6 mg/gm, respectively. Methanolic and water extracts of R. ellipticus had highest flavonoid concentration of 3.30 mg/gm and 2.86 mg/gm, respectively. Methanolic extract of R. ellipticus has highest antioxidant capacity, as measured by ABTS, DPPH, and FRAP tests, followed by the water extract for ABTS and FRAP value. Total flavonoid content (mg/gm) and ABTS (CEAC mg/ml) had a significant relationship. For HPLC analysis, a % methanolic fraction of REW with 29.49 mg/g TPC and a yield of 91.4% was used. The primary three phenolic components of REW70 fraction, quercetin, benzoic acid, and phenylacetic acid, were found to have quantities of 447 µg/ml, 143 µg/ml, and 103.3 µg/ml, respectively, according to HPLC analysis. Only two compounds in fractions REW70F3 and REW70F5, namely benzoic acid and quercetin, showed anticancer activity against HeLa cancer cells, according to cell viability assays for the three main compounds with fraction REW70F3, REW70F4, and REW70F5. REW70F3 and REW70F5 have IC50 values of 77.05083 and 137 µg/ml, respectively. The overexpression of DNMT1 and HDAC3 in REW70F3 treated HeLa cells was calculated by gene expression analysis in treated HeLa tumor cells, but only HDAC3 overexpression was estimated in REW70F5 compound. In case of REW70F5, DNMT1 was discovered to be underexpressed. In all samples, DNMT3A, DNMT3B, HDAC1 and HDAC2 genes were found to be underexpressed.