EFFECT OF ADJUVANTS ON MICROPROPAGATION OF TRICHODESMA INDICUM- A MEDICINAL HERB

Development of a reliable micropropagation protocol is necessary to facilitate shoot proliferation in Trichodesma indicum. The in vitro nodal segments were obtained from embryo explants collected from elite genotype and inoculated on Murashige and Skoog (MS) medium supplemented with 2.22 FM BAP. High frequency multiple shoot proliferation was achieved from differentiated shoots on MS medium fortified with 2.22 FM BAP and additives: adenine sulphate, citric acid, polyvinyl pyrroledone or activated charcoal. Among all the adjuvant, only PVP showed maximum shoot proliferation. About 700 shoots per culture were harvested within four months. Micropropagated shoots were successfully rooted on half MS medium with indole- 3- butyric acid (2.46 FM) for 72h pulse and then transferred to medium without IBA. The in vitro raised plantlets were successfully acclimatized and then established in the field, where they exhibited normal growth.