Cytotoxicity of Culture Filtrates of Aspergillus niger L. Derived from the Deproteinized Juice of Cabbage on Allium sativum L. Root Cells

DPJ is the byproduct of green crop fractionation and it was found appropriate for plant and fungi growth. It was found that DPJ from Lucerne cause abnormalities in onion root tips. In order to confirm the Aspergillus niger culture filtrate influence on meristematic root growth, mitosis of garlic was attempted. It was grown on the natural leafy extract at Botany Laboratory of Ruparel College, Mumbai. The protein was isolated from the juice, called as Deproteinized Juice (DPJ) as the medium, compared with Potato Dextrose Broth (PDB) as control medium. Growth of the fungi was supressed by all concentrations of DPJ. Grown fungal biomass culture filtrates of DPJ at various concentrations consumed to study its influence on cytology of Allium. The various concentrations resulted to root growth.The increased concentration of DPJ became responsible to cause the chromosomal abberations like clumping, stickiness, micronuclei etc especially in the stages of prophases. It concludes that culture filtrates of fungi grown on DPJ has the efficacy in changing the chromosomes by retarding the further cell division to metaphase. It indicates that the Aspergillus niger influenced as the antibiotic for Allium survival.