Isolation and Charecterization of Phytoconstituents from Annona reticulata L. Leaf Extracts

Aims: The present work was designed to isolate the chemical constituents from the leaf extracts of the plant Annona reticulata L. most commonly known as Ramphal or Bullock’s heart or Custard apple belongs to the family Annonaceae.

Methods: The extraction of leaf powder was done using various solvents. The preliminary phytochemical tests were carried out using standard chemical tests. Phytochemical constituents were isolated using column chromatography and characterization of the compounds were carried out using IR, 1H NMR, and mass spectroscopy data.

Results: The results from the preliminary phytochemical studies showed the presence of alkaloids, triterpenoids, carbohydrates,saponins and flavonoids. Isolation of leaf extracts revealed the presence of a steroid, alkaloid and flavonoid which were confirmed by spectral analysis to be α – amyrin caprylate, xylopine and fisetin.

Conclusion: The study confirmed the presence of α – Amyrin caprylate, Fisetin and Xylopine as its main chemical constituents.